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KMID : 0900920030270010069
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Korean journal of Animal Reproduction
2003 Volume.27 No. 1 p.69 ~ p.75
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Studies on the Efficient Embryo Transfer Methods using Inbred Embryos in Generation of Transgenic Mice
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Wang A.-G.
Kim S,-U.
Han Y.-H.
Kim S.-K.
Yu D.-Y.
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Abstract
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The objective of this study is to improve the efficiency of embryo transfer in generation of transgenic mice using inbred mouse (C57BL/6J) embryos. The embryos of C57BL/6J and BCF1 mice were superovulated by the standard protocol. One-cell stage of embryos were microinjected and the resulted one-or two-cell were transferred into one-or two-side oviducts of foster mother, respectively. When one-cell stage of embryos were transferred into one-side oviduct of 0.75 d.p.c. foster mother, the results were not ideal because of showing pregnancy ratios of 68.8pm7.83% for C57BL/6J and 48.3pm14.22% for BCF1, and development ratios of pups vs transferred embryos of 11.9pm5.51% for C57BL/6J and 10.5pm8.03% fur BCF1 . However, when two-cell stage of embryos were transferred into two-side oviducts of 0.5 d.p.c. foster mother, we got better results of 94.4pm9.64% and 100pm0% pregnancy ratio, and 22.1pm0.4% and 21.8pm0.38% development ratio for C57BL/6J and BCF1, respectively. Therefore, transferring two-cell stage of C57BL/6J embryos into two-side oviducts of 0.5 d.p.c. foster mother may be competitive to the result in BCF1 embryos. Even if there are a lot of other factors affecting these results, we conclude from these experiments that transfer of two-cell embryos into two-side oviducts of 0.5 d.p.c. foster mother is a more efficient and safe method than others in generating transgenic mice using inbred mice embryos.
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KEYWORD
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Inbred mouse, Embryo transfer, Superovulation
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